{"id":777,"date":"2018-11-01T10:34:18","date_gmt":"2018-11-01T10:34:18","guid":{"rendered":"http:\/\/www.drcocco.org\/?page_id=777"},"modified":"2020-03-18T10:22:12","modified_gmt":"2020-03-18T10:22:12","slug":"patents-and-publication","status":"publish","type":"page","link":"https:\/\/www.drcocco.org\/zh\/english-for-professionals\/bfgf-stem-cell-genesis\/patents-and-publication\/","title":{"rendered":"Patents and publications"},"content":{"rendered":"<h2 style=\"text-align: left;\"><u>Patents<\/u><\/h2>\n<p>&nbsp;<\/p>\n<ol>\n<li><strong>Wong, W.K.R.<\/strong> (2018) Means and method for expression of authentic human epidermal growth factor and\/or basic fibroblast growth factor in cytoplasm and\/or culture medium of <em>Escherichia coli<\/em>. Japanese patent No. 6404901. Granted on September 21, 2018.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"2\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam C.C., Ng, K.L. (2018) Novel Means and Method for production of hbFGF with <em>Bacillus subtilis<\/em>. Hong Kong Short-term Patent (under application).<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"3\">\n<li><strong>Wong, W.K.R.<\/strong>, Kwong, W. Y. (2017) Improved means and method for hyper-production of authentic human basic fibroblast growth factor in<em> Escherichia coli<\/em>. Hong Kong short-term patent No. HK1228651; Granted on November 3, 2017.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"4\">\n<li><strong>Wong, W.K.R.<\/strong> (2017) Means and method for expression of authentic human epidermal growth factor and\/or basic fibroblast growth factor in cytoplasm and\/or culture medium of <em>Escherichia coli<\/em> Hong Kong short-term patent No. HK1219200; Granted on March 24, 2017<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"5\">\n<li><strong>Wong, W.K.R.<\/strong> (2017) Means and method for expression of authentic human epidermal growth factor and\/or basic fibroblast growth factor in cytoplasm and\/or culture medium of <em>Escherichia coli<\/em> US Patent No. 9,580,484 B2; Granted on February 28, 2017.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"6\">\n<li><strong>Wong, W.K.R.<\/strong>, Ng, K. L. (2015) Means and method for enhancing growth of fish. Hong Kong shert-term patent No.: HK1218486; Granted on February 17, 2017.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"7\">\n<li><strong>Wong, W.K.R.<\/strong> (2015) Means and methods for producing authentic human basic fibroblast growth factor. US Patent: 9,017,968 B2; Granted on 28 April 2015.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"8\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. German Patent 60346186.7; Granted on 18 Jun 2014.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"9\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. PCT European Patent EP1482966B1; Granted on 14 May 2014.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"10\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. United Kingdom Patent EP1482966; Granted on 14 May 2014.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"11\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. French Patent EP1482966; Granted on 14 May 2014.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"12\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. Hong Kong Patent HK1071525; Granted on 20 May 2014.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"13\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2011) Method and composition for treating skin wounds with epidermal growth factor. Taiwan PCT National Phase Patent, Taiwanese Patent No.: I 348912. Granted on 11 Sep 2011<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"14\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2009) Method and composition for treating skin wounds with epidermal growth factor. China PCT National Phase Patent, Chinese Patent No. 3. Granted on 23 Dec 2009<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"15\">\n<li><strong>Wong, W.K.R.<\/strong>, Lam, K.H.E. and Tsang, M.W. (2009) Method and composition for treating skin wounds with epidermal growth factor. United States PCT National Phase Patent, US Patent No. 7,517,528 B2. Granted on 14 Apr 2009<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"16\">\n<li><strong>Wong, W.K.R.<\/strong> and Lam, K.H.E. (2000). Bacterial expression system. US Patent: 6,146,848. Granted on 14 Nov 2000<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"17\">\n<li><strong>Wong, W.K.<\/strong> and Chan, W.K. (2000). Cellobiase obtained from <em>Cellulomonas biazotea<\/em>. HK Patent: HK1012679. Granted on 09 Jun 2000<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"18\">\n<li><strong>Wong, W.K.<\/strong> and Chan, W.K. (1998). Cellobiase obtained from <em>Cellulomonas biazotea<\/em>. UK Patent: GB2 289 050 B.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"19\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. US Patent No. US 5646015 A<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"20\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. European Patent No. EP 0357 391 B1<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"21\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. Spanish Patent No.ES 2091197T3<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"22\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. German Patent No. EP 0357 391 B1<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"23\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. Canadian Patent No.CA 1,332,813C<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"24\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. USA Patent No. 5,223,407A<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"25\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. Japanese Patent No. JP 2177889A<\/li>\n<\/ol>\n<p><strong>\u00a0<\/strong><\/p>\n<ol start=\"26\">\n<li><strong>Wong, W.K.R.<\/strong> and Sutherland, M. (1990-1997). Excretion of heterologous proteins from <em> coli<\/em>. European Patent No. EP 357391A<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h2><u>Publications<\/u><\/h2>\n<p>&nbsp;<\/p>\n<ol>\n<li>Hu, X.H., Lai, C. Y. N., Sivakumar, T., Wang, H., Ng, K. L., Lam, C. C., and <strong>Wong, W. K. R<\/strong>. (2018). Novel strategy for expression of authentic and bioactive human basic fibroblast growth factor in <em>Bacillus subtilis<\/em>.\u00a0Applied microbiology and biotechnology,\u00a0102(16), 7061-7069.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"2\">\n<li><strong>Wong<\/strong><strong>, W. K. R<\/strong>.,\u00a0Ng, K. L. and Hu, X. H. (2018) Authentic Human Epidermal Growth Factor: A Panacea for Wound Healing.\u00a0EC Endocrinology and Metabolic Research4: 138-146.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"3\">\n<li>Sivakumar, T., Lai, C. Y. N., Lam, C. C., Hu, X. H., Wang, H., Ng, K. L., and <strong>Wong, W. K. R.<\/strong> (2018). Purification of authentic human basic fibroblast growth factor expressed in both the cytoplasm and culture medium of <em>Escherichia coli<\/em>.\u00a0Int J Chromatogr Sep Tech: IJCST-115.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"4\">\n<li>Chan, K.N. A., Ng, K.L.A., Ng, K.Y.K. and <strong>Wong, W.K.R<\/strong>. (2018) Cloning and characterization of two novel beta-glucosidase genes encoding isoenzymes of the cellobiase complex from <em>Cellulomonas biazotea<\/em>. Gene. 642: 367-375.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"5\">\n<li><strong>Wong, W. K. R<\/strong>., Ng, K. L., Lam, C.C., Hu, X.H., Lai, N.C.Y., Wang, H., and Sivakumar, T. (2017) Review Article: Reasons for Underrating the Potential of Human Epidermal Growth Factor in Medical Applications. J Anal Pharm Res 4(2): 00101. DOI: 10.15406\/japlr.2017.04.00101<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"6\">\n<li>Kwong, K. W. Y., T. Sivakumar, <strong>Wong, W. K. R.<\/strong> (2016) Intein mediated hyper-production of authentic human basic fibroblast growth factor in <em>Escherichia coli<\/em>. Scientific Reports. 6:33948; doi: 10.1038\/srep33948.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"7\">\n<li><strong>Wong, W. K. R.<\/strong>, Kwong, K. W. Y., Ng, K. L. (2016) Application of Recombinant Human Epidermal Growth Factor to Effective Treatment of Scalds. J Anal Pharm Res 3(1): 00045. DOI: 10.15406\/japlr.2016.03.00045.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"8\">\n<li>Kwong, K. W. Y., Ng, K. L., <strong>Wong, W. K. R.<\/strong> (2016) Engineering versatile protein expression systems mediated by inteins in <em>Escherichia coli<\/em>. Appl Microbiol Biotechnol. 100:255-262.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p style=\"padding-left: 30px;\">9. Ng, A.K.L., Lam, G.C.C., Kwong, K.W. Y., Dik, D.W.N., Lai, N.C.Y., Yong, J.L.W., Qian, P.Y.,<strong> Wong, W.K.R.<\/strong> (2016) Enhancement of fish growth employing feed\u00a0supplemented with recombinant fish growth hormone expressed in\u00a0<em>Bacillus subtilis<\/em>. Research Journal of Biotechnology. 11(1): 1-11<em>.<\/em><\/p>\n<p>&nbsp;<\/p>\n<ol start=\"10\">\n<li><strong>Wong, W.K.R.<\/strong> (2015) Effective Treatment of hard-to-heal surgical wound with recombinant human epidermal growth factor. Modern Chemistry &amp; Applications. 3(4):166, 1-3.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"11\">\n<li><strong>Wong, W.K.R.<\/strong> (2015) Recombinant production of human skin growth factor for effective treatments of hard-to-heal wounds. J. Diabetes Metab.<strong>6<\/strong> (8): 52.<\/li>\n<\/ol>\n<p><strong>\u00a0<\/strong><\/p>\n<ol start=\"12\">\n<li><strong>Wong, W.K.R. (<\/strong>May 2014) Means and methods for producing authentic human basic fibroblast growth factor. United States Patent Application, Pub. No.: 2014\/0134673 A1.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"13\">\n<li>Kwong, K. W. Y., <strong>Wong, W.K.R.<\/strong> (2013) A revolutionary approach facilitating co- expression of authentic human epidermal growth factor and basic fibroblast growth factor in both the cytoplasm and culture medium of <em>Escherichia coli<\/em>. Appl Microbiol Biotechnol. 97:9071-9080.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"14\">\n<li>Kwong, K. W.Y., Ng, A.K.L., Lam, C.C., Wang, Y.Y., <strong>Wong, W.K.R.<\/strong> (2013) Authentic human basic fibroblast growth factor produced by secretion in <em>Bacillus subtilis<\/em>. Appl Microbiol Biotechnol. <strong>97<\/strong>:6803-6811.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"15\">\n<li><strong>Wong, W.K.R.<\/strong>, Fu, B.Z.B., Wang, Y.Y., Ng, A.K.L. and Chan, A.K.N. (2012) Engineering of efficient <em>Escherichia coli <\/em>excretion systems for the production of heterologous proteins for commercial applications. Recent Patents on Chemical Engineering <strong>5<\/strong>:45:55 (Invited Review).<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"16\">\n<li>Chan, A.K.N., Wang, Y.Y., Ng, A.K.L., Fu, B.Z.B. and<strong> Wong, W.K.R. <\/strong>(2012) Cloning and characterization of a novel cellobiase gene, <em>cba3<\/em>, encoding the first known \u03b2-glucosidase of glycoside hydrolase family 1 of <em>Cellulomonas biazotea<\/em>. Gene <strong>493<\/strong>:52-61.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"17\">\n<li>Wang, Y.Y., Fu, B.Z.B., Ng, A.K.L., Lam, G.C.C., Chan, A.K.N., Sze, B.K.F. and<strong> Wong, W.K.R. <\/strong>(2011) Enhancement of excretory production of an exoglucanase from <em>Escherichia coli<\/em> with phage shock protein A (PspA) over-expression. J Microbiol Biotechnol. <strong>21<\/strong>:637-645.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"18\">\n<li>Wang, Y.Y., Ng, A.K.L., Lam, G.C.C., Chan, A.K.N., Sze, B.K.F., Fu, B.Z.B. and<strong> Wong, W.K.R. <\/strong>(2010) Efficient <em>Bacillus subtilis<\/em> promoters for graded expression of heterologous genes in <em>Escherichia coli<\/em>. Res. J. Biotech.<strong>5<\/strong>:5-14.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"19\">\n<li><strong>Wong, W.K.R.<\/strong> (2008) Application of recombinant microbial systems to the production of commercially valuable proteins. Res. J. Biotech. <strong>3<\/strong> (3): 3-4.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"20\">\n<li>K.L., Lam, C.C., Fu, Z.B., Han, Y.F., Tsim, K.W.K. and <strong>Wong, W.K.R.<\/strong> (2007)<em> Cloning and characterization of the yjeA gene, encoding a novel deoxyribonuclease, from Bacillus subtilis.<\/em> J. Biochem. <strong>142<\/strong>:647-654.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<ol start=\"21\">\n<li>Fu, Z.B. and Ng, K.L., Lam, C.C., Leung, K.C., Yip, W.H. and <strong>Wong, W.K.R. <\/strong>(2006) A two-stage refinement approach for the enhancement of excretory production of an exoglucanase from <em>Eshcerichia coli<\/em>. Protein Expression and Purification. Protein Expression and Purification <strong>48:<\/strong>205-214.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"22\">\n<li>Chen, X., Xu, Z., Cen, P. and <strong>Wong, W.K.R.<\/strong> (2006) Enhanced plasmid stability and production of hEGF by immobilized recombinant E. coli JM101. Biochemical Engineering Journal <strong>28<\/strong>:315-219.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"23\">\n<li>Fu, Z.B., Lam, T.L. and <strong>Wong, W.K.R. <\/strong>(2005) Cell death caused by \u201csaturated translocation\u201d of a secretory exoglucanase in <em>Escherichia coli<\/em>. Protein Expression and Purification <strong>42<\/strong>:67-77.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"24\">\n<li>Chen, X., Xu, Z., Cen, P. and <strong>Wong, W.K.R.<\/strong> (2005) A novel two-stage continuous process for excretive expression of hEGF by recombinant <em> coli<\/em> JM101. Process Biochemistry <strong>40<\/strong>:1-4.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"25\">\n<li>Tsang, M.W., Tsang, K.Y. and <strong>Wong, W.K.R. <\/strong>(2004) A case report on the use of recombinant human epidermal growth factor (rhEGF) in a gentlemen with drug induced Steven Johnson Syndrome. Derm. Ontline J. <strong>10<\/strong> (1): Article 25.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"26\">\n<li>Tsang, M.W., <strong>Wong, W.K.R<\/strong>., Hung, C.S., Lai, K.M., Tang, W., Cheung, E.Y.N., Kam, G., Leung. L., Chan, C.W., Chu, C.M. and Lam, E.K.H. (2003). Human epidermal growth factor enhances healing of diabetic foot ulcers. Diabetes Care <strong>26<\/strong>: 1856-61.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"27\">\n<li><strong>Wong, W.K.R<\/strong>., Ali, A.B. and Ma, M.C. (2003) Cloning, expression and characterization of diuretic hormone <em>Manduca<\/em> diuresin from <em>Manduca sexta<\/em> in <em>Escherichia coli<\/em>. Protein Expression and Purification <strong>29<\/strong>:51-57.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"28\">\n<li>Yu, J., Si, Y. and <strong>Wong, W.K.R.<\/strong> (2002). Kinetics modeling on inhibition and utilization of mixed volatile fatty acids in formation of polyhdroxyalkanoates by <em>Ralstonia eutropha<\/em>. Process Biochemistry <strong>37<\/strong>:731-738.<\/li>\n<\/ol>\n<p><strong>\u00a0<\/strong><\/p>\n<ol start=\"29\">\n<li>Tsang M.W., Hung, C.S., Cheung, E., Lai, K.M., Tang, W., Leung, L., Chan, C.S., Chu, C.M., Lam, E., <strong>Wong, W.K.R.<\/strong> (2002) Epidermal growth factor enhances healing of diabetics foot ulcer. Diabetes Research and Clinical Practice <strong>56<\/strong> (Suppl.1) S64-S65.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"30\">\n<li>Lau, A.T.Y. and <strong>Wong, W.K.R.<\/strong> (2001). Purification and characterization of a major secretory cellobiase, Cba2, from <em>Cellulomonas biazotea<\/em>. Protein Expression and Purification <strong>23<\/strong>:159-166.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"31\">\n<li>Xu, Z.H., Cen, P.L. and <strong>Wong, W.K.R.<\/strong> (2001) The effects of different glucose feeding modes on hEGF production in an excretory recombinant <em>Escherichia coli<\/em> K12 system. Chinese Journal of Biotechnology <strong>17<\/strong>:594-597.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"32\">\n<li><strong>Wong<\/strong>, <strong>K.R<\/strong>., Lam, E., Huang, R.C., Wong, R.S.C., Morris, C. and Hackett, J. (2001). Applications, and efficient large-scale production, of recombinant human epidermal growth factor. Invited for publication in Biotechnology &amp; Genetic Engineering Reviews <strong>18<\/strong>:51-71.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"33\">\n<li>Ma, M., Emery, S.B., <strong>Wong, W.K.R.<\/strong> and De Loof, A. (2000). Effects of <em>Manduca<\/em> diuresin on neonates of the tobacco hornworm, <em>Manduca sexta<\/em>. General and Comparative Endocrinology <strong>118<\/strong>:1-7.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"34\">\n<li>Xiang, Y., <strong>Wong, W.K.R.<\/strong>, Ma, M.C. and Wong, R.S.C. (2000). <em>Agrobacterium<\/em>-mediated transformation of <em>Brassica camprestris<\/em> <em>parachinensis<\/em> with synthetic <em>Bacillus thuringiensis cryIAb<\/em> and <em>cryIAc<\/em> genes. Plant Cell Reports <strong>19<\/strong>:251-256.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"35\">\n<li>Tong, L., Lin, Q., <strong>Wong, W.K.R.<\/strong>, Ali, A., Lim, D., Sung, W., Hew, C.L., and Yang, D.S.C. (2000). Extracellular expression, purification and characterization of a winter flounder antifreeze polypeptide from <em>Escherichia<\/em> Protein Expression and Purification <strong>18<\/strong>:175-181.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"36\">\n<li>Xu, Z., Liu, G., Cen, P. and <strong>Wong, W.K.R.<\/strong> (2000). Factors influencing excretive production of human epidermal growth factor (hEGF) with recombinant <em>Escherichia coli<\/em> K12 system. Bioprocess Engineering <strong>23:<\/strong>669-674.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"37\">\n<li>Huang, R.C., Lam, E., Chen, Y.H., Hackett, J., Lam, T.L., Liu, D., Ma, M.C., Siu, K.L., Sivakesava, S., Xu, Z.N., Wong, R.S.C. and <strong>Wong, W.K.R.<\/strong> (1999). Human epidermal growth factor excreted by recombinant <em>Escherichia coli<\/em> K-12 has the correct N-terminus and is fully bioactive. Process Biochemistry <strong>35<\/strong>:1-5.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"38\">\n<li>Sivakesava, S., Xu. Z.N., Chen, Y.H., Hackett, J., Huang, R.C., Lam, E., Lam, T.L., Siu, K.L., Wong, R.S.C., and <strong>Wong, W.K.R.<\/strong> (1999). Production of excreted human epidermal growth factor (hEGF) by an efficient recombinant <em>Escherichia coli<\/em>\u00a0 Process Biochemistry <strong>34<\/strong>:893-900.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"39\">\n<li>Lin, Q., Ewart, K.V., Yan, Q., <strong>Wong, W.K.R.<\/strong>, Yang, D.S.C. and Hew, C.L. (1999). Secretory expression and site-directed mutagenesis studies of the winter flounder skin-type antifreeze polypeptides. Eur. J. Biochem. <strong>264<\/strong>:49-54.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"40\">\n<li>Wong, D.K.H., Morris, C., Lam, T.L., <strong>Wong, W.K.R.<\/strong> and Hackett, J. (1999). Identification of O-antigen polymerase transcription and translation start signals and visualization of the protein in <em>Salmonella enterica<\/em> serovar <em>Typhimurium<\/em>. Microbiology <strong>145<\/strong>:2443-2451.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"41\">\n<li>Wong, D.K.H., Lam, K.H.E., Chan, C.K.P., Wong, Y.C.V., <strong>Wong, W.K.R.<\/strong> and Hackett, J. (1998). Extracellular expression of human epidermal growth factor encoded by an <em>Escherichia coli<\/em> K-12 plasmid stabilized by the <em>ytl2-incR<\/em> system system of <em>Salmonella typhimurium. <\/em> Industrial Microbiology &amp; Biotechnology <strong>21<\/strong>:31-36.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"42\">\n<li>Lam, K.H.E., Chow, K.C. and <strong>Wong, W.K.R.<\/strong> (1998). Construction of an efficient <em>Bacillus subtilis<\/em> system for extracellular production of heterologous proteins. J. Biotechnol. <strong>63<\/strong>:167-177.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"43\">\n<li><strong>Wong W.K.R.<\/strong>, Ali, A., Chan, W.K., Ho, V. and Lee, N.T.K. (1998). The cloning, expression and characterization of a cellobiase gene encoding a secretory enzyme from <em>Cellulomonas biazotea.<\/em> Gene <strong>207<\/strong>: 79-86.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"44\">\n<li>Lam, T.L., Wong, R.S.C. and <strong>Wong, W.K.R.<\/strong> (1997). Enhancement of extracellular production of a <em>Cellulomonas fimi<\/em> exoglucanase in <em>Escherichia coli<\/em> by the reduction of promoter strength. Enzyme Microb. Technol. <strong>20<\/strong>:482-488.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"45\">\n<li>Liu, D., Yu, Y., <strong>Wong, W.K.R.<\/strong>, Zhao, X. and Zhang, J. (1997). An Enzyme-like polyclonal antibody capable of catalyzing ester hydrolysis. Enzyme Microb. Technol. <strong>20<\/strong>:24-31.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"46\">\n<li>Emery, S.B., Ma, M.C., <strong>Wong, W.K.R.<\/strong>, Tips, A. and De Loof, A. (1994). Immunocytochemical localization of a diuretic peptide <em>Manduca<\/em> diuresin (MasDPII) in the brain and suboesophageal ganglion of the tobacco hawkmoth <em>Manduca sexta.<\/em> Insect Biochem. Physiol. <strong>27<\/strong>:137-152.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"47\">\n<li>Rabbani, S.A., <strong>Wong, R.<\/strong>, Kronis, K.A., Henderson, H., Rajwans, N., Bozzato, R.P., Hendy, G.N. and Goltzman, D. (1991). Expression and characterization of recombinant human parathyroid hormone-like peptide using a bacterial excretion system. J. Bone Min. Res. <strong>6<\/strong> (Supplement 1): Abstract 133, S116.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"48\">\n<li><strong>Wong, W.K.R.<\/strong>, Curry, C., Parekh, R.S., Parekh, S.R., Wayman, M., Davies, R.W. Kilburn, D.G. and Skipper, N. (1988). Wood hydrolysis by <em>Cellulomonas fimi<\/em> endoglucanase and exoglucanase coexpressed as secreted enzymes in <em>Saccharomyces<\/em> <em>cerevisiae<\/em>. Nature Biotechnology <strong>6<\/strong>:713-719.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"49\">\n<li>Skipper, N., Bozzato, R.P., Vetter, D., Davies, R.W., <strong>Wong, R.<\/strong> and Hopper, J.E. (1987). Use of the melibiase promoter and signal peptide to express a bacterial cellulase from yeast, p.135-147. In: Biological Research on Industrial Yeasts, Vol. 1. Stewart, G.G., Russell, I., Klein, R.D., and Heibsch, R.R. (Eds.). CRC Press, Boca Raton, Florida.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"50\">\n<li>Warren, R.A.J., Beck, C.F., Gilkes, N.R., Kilburn, D.G., Langsford, M.L., Miller, R.C. Jr., O&#8217;Neill, G.P. and <strong>Wong, W.K.R.<\/strong> (1987). Unusual structural organization of two cellulases from <em>Cellulomonas fimi<\/em>, p.261-266. Chapter 28 in: Wood and Cellulosics: Industrial Utilisation, Biotechnology, Structure and Properties. Kennedy, J.F., Phillips, G.O., and Williams, P.A. (Eds.). Ellis Horwood Limited, Chichester.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"51\">\n<li><strong>Wong, W.K.R.<\/strong>, Gerhard, B., Guo, Z.M., Kilburn, D.G., Warren, R.A.J. and Miller, R.C. Jr. (1986). Characterization and structure of an endoglucanase gene <em>cenA<\/em> of <em>Cellulomonas fimi<\/em>. Gene <strong>44<\/strong>:315-324.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"52\">\n<li>Johnson, J.A., <strong>Wong, W.K.R.<\/strong> and Beatty, J.T. (1986). Expression of cellulase genes in <em>Rhodobacter capulatus<\/em> by use of plasmid expression vectors. J. Bacteriol. <strong>167<\/strong>:604-610.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"53\">\n<li>Warren, R.A.J., Beck, C.F., Gilkes, N.R., Kilburn, D.G., Langsford, M.L., Miller, C. Jr., O&#8217;Neill, G.P., Scheufens, M. and <strong>Wong, W.K.R.<\/strong> (1986). Sequence conservation and region shuffling in an endoglucanase and an exoglucanase from <em>Cellulomonas fimi<\/em>. Proteins <strong>1<\/strong>:335-341.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"54\">\n<li>Skipper, N., Sutherland, M., Davies, R.W., Kilburn, D., Miller, R.C. Jr., Warren, A. and <strong>Wong, R.<\/strong> (1985). Secretion of a bacterial cellulase by yeast. Science <strong>230<\/strong>:958-960.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"55\">\n<li>Gilkes, N.R., Langsford, M.L., Kilburn, D.G., Miller, Jr., R.C., Wakarchuk, W.W., Warren, R.A.J., Whittle, D.J. and <strong>Wong W.K.R.<\/strong> (1984). Isolation and characterization of <em>Escherichia coli<\/em> clones expressing cellulase genes from <em>Cellulomonas fimi<\/em>. J. Gen. Microbiol. <strong>130<\/strong>:1377-1384.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<ol start=\"56\">\n<li>Gilkes, N.R., Kilburn, D.G., Langsford, M.L., Miller, Jr., R.C., O&#8217;Neil, G.P., Owolabi, J.B., Wakarchuk, W.W., Warren, R.A.J., Whittle, D.J. and <strong>Wong W.K.R.<\/strong> (1984). The cloning of <em>Cellulomonas fimi<\/em> cellulase genes in <em>Escherichia coli<\/em>. In: Fifth Canadian Bioenergy Research and Development Seminar, p. 582. Hasnain, S. (ed.). Elsevier Applied Science Publishers, New York.<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>Patents &nbsp; Wong, W.K.R. (2018) Means and method for expression of authentic human epidermal growth factor and\/or basic fibroblast growth factor in cytoplasm and\/or culture medium of Escherichia coli. Japanese patent No. 6404901. Granted on September 21, 2018. &nbsp; Wong, W.K.R., Lam C.C., Ng, K.L. (2018) Novel Means and Method for production of hbFGF with&#8230;<\/p>","protected":false},"author":1,"featured_media":0,"parent":606,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/pages\/777"}],"collection":[{"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/comments?post=777"}],"version-history":[{"count":7,"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/pages\/777\/revisions"}],"predecessor-version":[{"id":1016,"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/pages\/777\/revisions\/1016"}],"up":[{"embeddable":true,"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/pages\/606"}],"wp:attachment":[{"href":"https:\/\/www.drcocco.org\/zh\/wp-json\/wp\/v2\/media?parent=777"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}