專利及文獻

歐、美、中、港、台、日六大專利

Patents

 

  1. Wong, W.K.R. (2018) Means and method for expression of authentic human epidermal growth factor and/or basic fibroblast growth factor in cytoplasm and/or culture medium of Escherichia coli. Japanese patent No. 6404901. Granted on September 21, 2018.

 

  1. Wong, W.K.R., Lam C.C., Ng, K.L. (2018) Novel Means and Method for production of hbFGF with Bacillus subtilis. Hong Kong Short-term Patent (under application).

 

  1. Wong, W.K.R., Kwong, W. Y. (2017) Improved means and method for hyper-production of authentic human basic fibroblast growth factor in Escherichia coli. Hong Kong short-term patent No. HK1228651; Granted on November 3, 2017.

 

  1. Wong, W.K.R. (2017) Means and method for expression of authentic human epidermal growth factor and/or basic fibroblast growth factor in cytoplasm and/or culture medium of Escherichia coli Hong Kong short-term patent No. HK1219200; Granted on March 24, 2017

 

  1. Wong, W.K.R. (2017) Means and method for expression of authentic human epidermal growth factor and/or basic fibroblast growth factor in cytoplasm and/or culture medium of Escherichia coli US Patent No. 9,580,484 B2; Granted on February 28, 2017.

 

  1. Wong, W.K.R., Ng, K. L. (2015) Means and method for enhancing growth of fish. Hong Kong shert-term patent No.: HK1218486; Granted on February 17, 2017.

 

  1. Wong, W.K.R. (2015) Means and methods for producing authentic human basic fibroblast growth factor. US Patent: 9,017,968 B2; Granted on 28 April 2015.

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. German Patent 60346186.7; Granted on 18 Jun 2014.

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. PCT European Patent EP1482966B1; Granted on 14 May 2014.

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. United Kingdom Patent EP1482966; Granted on 14 May 2014.

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. French Patent EP1482966; Granted on 14 May 2014.

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. Hong Kong Patent HK1071525; Granted on 20 May 2014.

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2011) Method and composition for treating skin wounds with epidermal growth factor. Taiwan PCT National Phase Patent, Taiwanese Patent No.: I 348912. Granted on 11 Sep 2011

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2009) Method and composition for treating skin wounds with epidermal growth factor. China PCT National Phase Patent, Chinese Patent No. 3. Granted on 23 Dec 2009

 

  1. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2009) Method and composition for treating skin wounds with epidermal growth factor. United States PCT National Phase Patent, US Patent No. 7,517,528 B2. Granted on 14 Apr 2009

 

  1. Wong, W.K.R. and Lam, K.H.E. (2000). Bacterial expression system. US Patent: 6,146,848. Granted on 14 Nov 2000

 

  1. Wong, W.K. and Chan, W.K. (2000). Cellobiase obtained from Cellulomonas biazotea. HK Patent: HK1012679. Granted on 09 Jun 2000

 

  1. Wong, W.K. and Chan, W.K. (1998). Cellobiase obtained from Cellulomonas biazotea. UK Patent: GB2 289 050 B.

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. US Patent No. US 5646015 A

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. European Patent No. EP 0357 391 B1

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. Spanish Patent No.ES 2091197T3

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. German Patent No. EP 0357 391 B1

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. Canadian Patent No.CA 1,332,813C

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. USA Patent No. 5,223,407A

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. Japanese Patent No. JP 2177889A

 

  1. Wong, W.K.R. and Sutherland, M. (1990-1997). Excretion of heterologous proteins from coli. European Patent No. EP 357391A

 

 

 

Publications

 

  1. Hu, X.H., Lai, C. Y. N., Sivakumar, T., Wang, H., Ng, K. L., Lam, C. C., and Wong, W. K. R. (2018). Novel strategy for expression of authentic and bioactive human basic fibroblast growth factor in Bacillus subtilis. Applied microbiology and biotechnology, 102(16), 7061-7069.

 

  1. Wong, W. K. R., Ng, K. L. and Hu, X. H. (2018) Authentic Human Epidermal Growth Factor: A Panacea for Wound Healing. EC Endocrinology and Metabolic Research4: 138-146.

 

  1. Sivakumar, T., Lai, C. Y. N., Lam, C. C., Hu, X. H., Wang, H., Ng, K. L., and Wong, W. K. R. (2018). Purification of authentic human basic fibroblast growth factor expressed in both the cytoplasm and culture medium of Escherichia coli. Int J Chromatogr Sep Tech: IJCST-115.

 

  1. Chan, K.N. A., Ng, K.L.A., Ng, K.Y.K. and Wong, W.K.R. (2018) Cloning and characterization of two novel beta-glucosidase genes encoding isoenzymes of the cellobiase complex from Cellulomonas biazotea. Gene. 642: 367-375.

 

  1. Wong, W. K. R., Ng, K. L., Lam, C.C., Hu, X.H., Lai, N.C.Y., Wang, H., and Sivakumar, T. (2017) Review Article: Reasons for Underrating the Potential of Human Epidermal Growth Factor in Medical Applications. J Anal Pharm Res 4(2): 00101. DOI: 10.15406/japlr.2017.04.00101

 

  1. Kwong, K. W. Y., T. Sivakumar, Wong, W. K. R. (2016) Intein mediated hyper-production of authentic human basic fibroblast growth factor in Escherichia coli. Scientific Reports. 6:33948; doi: 10.1038/srep33948.

 

  1. Wong, W. K. R., Kwong, K. W. Y., Ng, K. L. (2016) Application of Recombinant Human Epidermal Growth Factor to Effective Treatment of Scalds. J Anal Pharm Res 3(1): 00045. DOI: 10.15406/japlr.2016.03.00045.

 

  1. Kwong, K. W. Y., Ng, K. L., Wong, W. K. R. (2016) Engineering versatile protein expression systems mediated by inteins in Escherichia coli. Appl Microbiol Biotechnol. 100:255-262.

 

9. Ng, A.K.L., Lam, G.C.C., Kwong, K.W. Y., Dik, D.W.N., Lai, N.C.Y., Yong, J.L.W., Qian, P.Y., Wong, W.K.R.(2016) Enhancement of fish growth employing feed supplemented with recombinant fish growth hormone expressed in Bacillus subtilis. Research Journal of Biotechnology. 11(1): 1-11.

 

  1. Wong, W.K.R. (2015) Effective Treatment of hard-to-heal surgical wound with recombinant human epidermal growth factor. Modern Chemistry & Applications. 3(4):166, 1-3.

 

  1. Wong, W.K.R. (2015) Recombinant production of human skin growth factor for effective treatments of hard-to-heal wounds. J. Diabetes Metab.6 (8): 52.

 

  1. Wong, W.K.R. (May 2014) Means and methods for producing authentic human basic fibroblast growth factor. United States Patent Application, Pub. No.: 2014/0134673 A1.

 

  1. Kwong, K. W. Y., Wong, W.K.R. (2013) A revolutionary approach facilitating co- expression of authentic human epidermal growth factor and basic fibroblast growth factor in both the cytoplasm and culture medium of Escherichia coli. Appl Microbiol Biotechnol. 97:9071-9080.

 

  1. Kwong, K. W.Y., Ng, A.K.L., Lam, C.C., Wang, Y.Y., Wong, W.K.R. (2013) Authentic human basic fibroblast growth factor produced by secretion in Bacillus subtilis. Appl Microbiol Biotechnol. 97:6803-6811.

 

  1. Wong, W.K.R., Fu, B.Z.B., Wang, Y.Y., Ng, A.K.L. and Chan, A.K.N. (2012) Engineering of efficient Escherichia coli excretion systems for the production of heterologous proteins for commercial applications. Recent Patents on Chemical Engineering 5:45:55 (Invited Review).

 

  1. Chan, A.K.N., Wang, Y.Y., Ng, A.K.L., Fu, B.Z.B. and Wong, W.K.R. (2012) Cloning and characterization of a novel cellobiase gene, cba3, encoding the first known β-glucosidase of glycoside hydrolase family 1 of Cellulomonas biazotea. Gene 493:52-61.

 

  1. Wang, Y.Y., Fu, B.Z.B., Ng, A.K.L., Lam, G.C.C., Chan, A.K.N., Sze, B.K.F. and Wong, W.K.R. (2011) Enhancement of excretory production of an exoglucanase from Escherichia coli with phage shock protein A (PspA) over-expression. J Microbiol Biotechnol. 21:637-645.

 

  1. Wang, Y.Y., Ng, A.K.L., Lam, G.C.C., Chan, A.K.N., Sze, B.K.F., Fu, B.Z.B. and Wong, W.K.R. (2010) Efficient Bacillus subtilis promoters for graded expression of heterologous genes in Escherichia coli. Res. J. Biotech.5:5-14.

 

  1. Wong, W.K.R. (2008) Application of recombinant microbial systems to the production of commercially valuable proteins. Res. J. Biotech. 3 (3): 3-4.

 

  1. K.L., Lam, C.C., Fu, Z.B., Han, Y.F., Tsim, K.W.K. and Wong, W.K.R. (2007) Cloning and characterization of the yjeA gene, encoding a novel deoxyribonuclease, from Bacillus subtilis. J. Biochem. 142:647-654.

 

 

  1. Fu, Z.B. and Ng, K.L., Lam, C.C., Leung, K.C., Yip, W.H. and Wong, W.K.R. (2006) A two-stage refinement approach for the enhancement of excretory production of an exoglucanase from Eshcerichia coli. Protein Expression and Purification. Protein Expression and Purification 48:205-214.

 

  1. Chen, X., Xu, Z., Cen, P. and Wong, W.K.R. (2006) Enhanced plasmid stability and production of hEGF by immobilized recombinant E. coli JM101. Biochemical Engineering Journal 28:315-219.

 

  1. Fu, Z.B., Lam, T.L. and Wong, W.K.R. (2005) Cell death caused by “saturated translocation” of a secretory exoglucanase in Escherichia coli. Protein Expression and Purification 42:67-77.

 

  1. Chen, X., Xu, Z., Cen, P. and Wong, W.K.R. (2005) A novel two-stage continuous process for excretive expression of hEGF by recombinant coli JM101. Process Biochemistry 40:1-4.

 

  1. Tsang, M.W., Tsang, K.Y. and Wong, W.K.R. (2004) A case report on the use of recombinant human epidermal growth factor (rhEGF) in a gentlemen with drug induced Steven Johnson Syndrome. Derm. Ontline J. 10 (1): Article 25.

 

  1. Tsang, M.W., Wong, W.K.R., Hung, C.S., Lai, K.M., Tang, W., Cheung, E.Y.N., Kam, G., Leung. L., Chan, C.W., Chu, C.M. and Lam, E.K.H. (2003). Human epidermal growth factor enhances healing of diabetic foot ulcers. Diabetes Care 26: 1856-61.

 

  1. Wong, W.K.R., Ali, A.B. and Ma, M.C. (2003) Cloning, expression and characterization of diuretic hormone Manduca diuresin from Manduca sexta in Escherichia coli. Protein Expression and Purification 29:51-57.

 

  1. Yu, J., Si, Y. and Wong, W.K.R. (2002). Kinetics modeling on inhibition and utilization of mixed volatile fatty acids in formation of polyhdroxyalkanoates by Ralstonia eutropha. Process Biochemistry 37:731-738.

 

  1. Tsang M.W., Hung, C.S., Cheung, E., Lai, K.M., Tang, W., Leung, L., Chan, C.S., Chu, C.M., Lam, E., Wong, W.K.R. (2002) Epidermal growth factor enhances healing of diabetics foot ulcer. Diabetes Research and Clinical Practice 56 (Suppl.1) S64-S65.

 

  1. Lau, A.T.Y. and Wong, W.K.R. (2001). Purification and characterization of a major secretory cellobiase, Cba2, from Cellulomonas biazotea. Protein Expression and Purification 23:159-166.

 

  1. Xu, Z.H., Cen, P.L. and Wong, W.K.R. (2001) The effects of different glucose feeding modes on hEGF production in an excretory recombinant Escherichia coli K12 system. Chinese Journal of Biotechnology 17:594-597.

 

  1. Wong, K.R., Lam, E., Huang, R.C., Wong, R.S.C., Morris, C. and Hackett, J. (2001). Applications, and efficient large-scale production, of recombinant human epidermal growth factor. Invited for publication in Biotechnology & Genetic Engineering Reviews 18:51-71.

 

  1. Ma, M., Emery, S.B., Wong, W.K.R. and De Loof, A. (2000). Effects of Manduca diuresin on neonates of the tobacco hornworm, Manduca sexta. General and Comparative Endocrinology 118:1-7.

 

  1. Xiang, Y., Wong, W.K.R., Ma, M.C. and Wong, R.S.C. (2000). Agrobacterium-mediated transformation of Brassica camprestris parachinensis with synthetic Bacillus thuringiensis cryIAb and cryIAc genes. Plant Cell Reports 19:251-256.

 

  1. Tong, L., Lin, Q., Wong, W.K.R., Ali, A., Lim, D., Sung, W., Hew, C.L., and Yang, D.S.C. (2000). Extracellular expression, purification and characterization of a winter flounder antifreeze polypeptide from Escherichia Protein Expression and Purification 18:175-181.

 

  1. Xu, Z., Liu, G., Cen, P. and Wong, W.K.R. (2000). Factors influencing excretive production of human epidermal growth factor (hEGF) with recombinant Escherichia coli K12 system. Bioprocess Engineering 23:669-674.

 

  1. Huang, R.C., Lam, E., Chen, Y.H., Hackett, J., Lam, T.L., Liu, D., Ma, M.C., Siu, K.L., Sivakesava, S., Xu, Z.N., Wong, R.S.C. and Wong, W.K.R. (1999). Human epidermal growth factor excreted by recombinant Escherichia coli K-12 has the correct N-terminus and is fully bioactive. Process Biochemistry 35:1-5.

 

  1. Sivakesava, S., Xu. Z.N., Chen, Y.H., Hackett, J., Huang, R.C., Lam, E., Lam, T.L., Siu, K.L., Wong, R.S.C., and Wong, W.K.R. (1999). Production of excreted human epidermal growth factor (hEGF) by an efficient recombinant Escherichia coli  Process Biochemistry 34:893-900.

 

  1. Lin, Q., Ewart, K.V., Yan, Q., Wong, W.K.R., Yang, D.S.C. and Hew, C.L. (1999). Secretory expression and site-directed mutagenesis studies of the winter flounder skin-type antifreeze polypeptides. Eur. J. Biochem. 264:49-54.

 

  1. Wong, D.K.H., Morris, C., Lam, T.L., Wong, W.K.R. and Hackett, J. (1999). Identification of O-antigen polymerase transcription and translation start signals and visualization of the protein in Salmonella enterica serovar Typhimurium. Microbiology 145:2443-2451.

 

  1. Wong, D.K.H., Lam, K.H.E., Chan, C.K.P., Wong, Y.C.V., Wong, W.K.R. and Hackett, J. (1998). Extracellular expression of human epidermal growth factor encoded by an Escherichia coli K-12 plasmid stabilized by the ytl2-incR system system of Salmonella typhimurium. Industrial Microbiology & Biotechnology 21:31-36.

 

  1. Lam, K.H.E., Chow, K.C. and Wong, W.K.R. (1998). Construction of an efficient Bacillus subtilissystem for extracellular production of heterologous proteins. J. Biotechnol. 63:167-177.

 

  1. Wong W.K.R., Ali, A., Chan, W.K., Ho, V. and Lee, N.T.K. (1998). The cloning, expression and characterization of a cellobiase gene encoding a secretory enzyme from Cellulomonas biazotea.Gene 207: 79-86.

 

  1. Lam, T.L., Wong, R.S.C. and Wong, W.K.R. (1997). Enhancement of extracellular production of a Cellulomonas fimi exoglucanase in Escherichia coli by the reduction of promoter strength. Enzyme Microb. Technol. 20:482-488.

 

  1. Liu, D., Yu, Y., Wong, W.K.R., Zhao, X. and Zhang, J. (1997). An Enzyme-like polyclonal antibody capable of catalyzing ester hydrolysis. Enzyme Microb. Technol. 20:24-31.

 

  1. Emery, S.B., Ma, M.C., Wong, W.K.R., Tips, A. and De Loof, A. (1994). Immunocytochemical localization of a diuretic peptide Manduca diuresin (MasDPII) in the brain and suboesophageal ganglion of the tobacco hawkmoth Manduca sexta. Insect Biochem. Physiol. 27:137-152.

 

  1. Rabbani, S.A., Wong, R., Kronis, K.A., Henderson, H., Rajwans, N., Bozzato, R.P., Hendy, G.N. and Goltzman, D. (1991). Expression and characterization of recombinant human parathyroid hormone-like peptide using a bacterial excretion system. J. Bone Min. Res. 6 (Supplement 1): Abstract 133, S116.

 

  1. Wong, W.K.R., Curry, C., Parekh, R.S., Parekh, S.R., Wayman, M., Davies, R.W. Kilburn, D.G. and Skipper, N. (1988). Wood hydrolysis by Cellulomonas fimi endoglucanase and exoglucanase coexpressed as secreted enzymes in Saccharomyces cerevisiae. Nature Biotechnology 6:713-719.

 

  1. Skipper, N., Bozzato, R.P., Vetter, D., Davies, R.W., Wong, R. and Hopper, J.E. (1987). Use of the melibiase promoter and signal peptide to express a bacterial cellulase from yeast, p.135-147. In: Biological Research on Industrial Yeasts, Vol. 1. Stewart, G.G., Russell, I., Klein, R.D., and Heibsch, R.R. (Eds.). CRC Press, Boca Raton, Florida.

 

  1. Warren, R.A.J., Beck, C.F., Gilkes, N.R., Kilburn, D.G., Langsford, M.L., Miller, R.C. Jr., O’Neill, G.P. and Wong, W.K.R. (1987). Unusual structural organization of two cellulases from Cellulomonas fimi, p.261-266. Chapter 28 in: Wood and Cellulosics: Industrial Utilisation, Biotechnology, Structure and Properties. Kennedy, J.F., Phillips, G.O., and Williams, P.A. (Eds.). Ellis Horwood Limited, Chichester.

 

  1. Wong, W.K.R., Gerhard, B., Guo, Z.M., Kilburn, D.G., Warren, R.A.J. and Miller, R.C. Jr. (1986). Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi. Gene 44:315-324.

 

  1. Johnson, J.A., Wong, W.K.R. and Beatty, J.T. (1986). Expression of cellulase genes in Rhodobacter capulatus by use of plasmid expression vectors. J. Bacteriol. 167:604-610.

 

  1. Warren, R.A.J., Beck, C.F., Gilkes, N.R., Kilburn, D.G., Langsford, M.L., Miller, C. Jr., O’Neill, G.P., Scheufens, M. and Wong, W.K.R. (1986). Sequence conservation and region shuffling in an endoglucanase and an exoglucanase from Cellulomonas fimi. Proteins 1:335-341.

 

  1. Skipper, N., Sutherland, M., Davies, R.W., Kilburn, D., Miller, R.C. Jr., Warren, A. and Wong, R. (1985). Secretion of a bacterial cellulase by yeast. Science 230:958-960.

 

  1. Gilkes, N.R., Langsford, M.L., Kilburn, D.G., Miller, Jr., R.C., Wakarchuk, W.W., Warren, R.A.J., Whittle, D.J. and Wong W.K.R. (1984). Isolation and characterization of Escherichia coli clones expressing cellulase genes from Cellulomonas fimi. J. Gen. Microbiol. 130:1377-1384.

 

  1. Gilkes, N.R., Kilburn, D.G., Langsford, M.L., Miller, Jr., R.C., O’Neil, G.P., Owolabi, J.B., Wakarchuk, W.W., Warren, R.A.J., Whittle, D.J. and Wong W.K.R. (1984). The cloning of Cellulomonas fimicellulase genes in Escherichia coli. In: Fifth Canadian Bioenergy Research and Development Seminar, p. 582. Hasnain, S. (ed.). Elsevier Applied Science Publishers, New York.